产品介绍 品名 Monoclonal Antibody to Human C5 货号 A217 规格 100 µL 同种型 IgG1k 蛋白质浓度 > 1.0 mg/mL 纯度 ≥ 95% by SDS PAGE 缓冲液 Borate Buffered Saline (pH 8.4 ± 0.2) 物种交叉反应 Human, baboon 储存 torage: ≤ 30 Days 2-8˚C > 30 Days ≤ –20˚C 使用说明 For Research Use Only. Not for use in diagnostic procedures 产品描述 A murine monoclonal antibody to an epitope on C5. This monoclonal antibody was raised against purified human C. It is specific for an antigen expressed on the C5 but not on MAC Complex. This antibody inhibits the lysis of EA and will not bind to MAC bound to a membrane. Quidel's C5 monoclonal antibody has been used in a variety of experimental techniques including EIA, Western Blot, and RIA 特异性 The specificity of the monoclonal antibody was established by ELISA and radioimmunoassay (RIA). Using RIA, this monoclonal antibody was shown to bind radiolabeled C5 protein. Specificity was confirmed by functional inhibition of C5. In this study, ~17 ng of the Anti-Human C5 monoclonal antibody gave 50% inhibition of the lysis of antibody-coated sheep erythrocytes (EA) in an assay designed to measure C5 hemolytic function. The assay used highly purified C5 protein, EA and normal human serum which was immunochemically depleted of C5. The antibody does not recognize TCC deposited on membranes. 应用 参考资料 1、Sandoval, A., Rong A., et al. Distal recognition site for classical pathway convertase located in the C345C/Netrin module of complement component C5, J. Immunol 165:2 1066-1073., 2000. 2、Barbashov, S. Wang, C., Nicholson-Weller, A. Serum amyloid P component forms a stable complex with human C5b-6, J. Immunol. 158:3830-3835, 1997.
产品介绍
Monoclonal Antibody to Human C5
A217
100 µL
IgG1k
> 1.0 mg/mL
≥ 95% by SDS PAGE
Borate Buffered Saline (pH 8.4 ± 0.2)
Human, baboon
torage: ≤ 30 Days 2-8˚C
> 30 Days ≤ –20˚C
For Research Use Only. Not for use in diagnostic procedures
A murine monoclonal antibody to an epitope on C5.
This monoclonal antibody was raised against purified human C. It is specific for an antigen expressed on the C5 but not on MAC Complex. This antibody inhibits the lysis of EA and will not bind to MAC bound to a membrane.
Quidel's C5 monoclonal antibody has been used in a variety of experimental techniques including EIA, Western Blot, and RIA
The specificity of the monoclonal antibody was established by ELISA and radioimmunoassay (RIA). Using RIA, this monoclonal antibody was shown to bind radiolabeled C5 protein. Specificity was confirmed by functional inhibition of C5. In this study, ~17 ng of the Anti-Human C5 monoclonal antibody gave 50% inhibition of the lysis of antibody-coated sheep erythrocytes (EA) in an assay designed to measure C5 hemolytic function. The assay used highly purified C5 protein, EA and normal human serum which was immunochemically depleted of C5. The antibody does not recognize TCC deposited on membranes.
1、Sandoval, A., Rong A., et al. Distal recognition site for classical pathway convertase located in the C345C/Netrin module of complement component C5, J. Immunol 165:2 1066-1073., 2000.
2、Barbashov, S. Wang, C., Nicholson-Weller, A. Serum amyloid P component forms a stable complex with human C5b-6, J. Immunol. 158:3830-3835, 1997.
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